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本文使用国产载脂蛋白AI和B试剂以TECHNICON RA系列自动生化分析仪进行透射免疫比浊分析法测定,并对实验条件的选择,方法性能等作了评价。结果表明,载脂蛋白AI及B平均回收率分别为98.8～101.1％和94.6～102.8％;载脂蛋白AI和B的批内最大CV％分别为2.17％和3.45％;载脂蛋白AI测定线性范围为0.61～4.27G/L,而载脂蛋白B为0.36～2.52G/L。实践证明,用本法测定载脂蛋白具有快速简便,结果准确,试剂和样品用量少,可同时测定批量标本等优点。
In this paper, the domestic apolipoprotein AI and B reagent to TECHNICON RA series of automatic biochemical analyzer for transmission immunoassay turbidimetric assay, and the choice of experimental conditions, method performance were evaluated. The results showed that the average recoveries of apolipoprotein AI and B were 98.8 ~ 101.1% and 94.6 ~ 102.8%, respectively; the maximum intra-assay CV contents of apolipoprotein AI and B were 2.17% and 3.45% respectively; The range was 0.61 to 4.27 G / L, while apolipoprotein B was 0.36 to 2.52 G / L. Practice has proved that the determination of apolipoprotein with this method is fast and easy, accurate results, reagent and sample dosage less, batch determination of the advantages of the same time.