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目的研究连翘苷在大鼠小肠的吸收特性。方法采用外翻肠囊法建立连翘苷大鼠小肠吸收模型,HPLC测定连翘苷的含量,分别进行大鼠小肠的十二指肠、空肠、回肠3个肠段、不同浓度吸收特性研究,以及P-糖蛋白(P-gp)抑制剂盐酸维拉帕米和吸收促进剂吐温-80对连翘苷吸收的影响研究。结果连翘苷浓度为10,20,40μg.mL-1 3个浓度时,吸收速度常数ka无显著性差异(P>0.05);在不同肠段中的吸收,通过量由多到少依次为回肠>空肠>十二指肠;加入盐酸维拉帕米和吐温-80的小组与正常组相比,吸收速度常数ka无显著性差异(P>0.05)。结论在试验剂量范围内,连翘苷的吸收呈一级动力学过程,吸收机制主要为被动扩散;连翘苷不是P-糖蛋白的底物。
Objective To study the absorption characteristics of forsythin in rat small intestine. Methods The intestinal absorption model of forsythin in rats was established by valgus gut method. Forsythin was determined by HPLC. The intestine of duodenum, jejunum and ileum of intestine were collected, and their absorption characteristics were studied. And P-glycoprotein (P-gp) inhibitor verapamil and absorption accelerator Tween -80 on the absorption of forsythin. Results When the concentration of forsythin was 10, 20 and 40 μg.mL-1, the absorption rate constant, ka, had no significant difference (P> 0.05). The absorption, Ileum> jejunum> duodenum. Compared with the normal group, the rate constant ka of verapamil hydrochloride and tween-80 group had no significant difference (p> 0.05). Conclusions Forsythin is first-order kinetic in the experimental dose range and the absorption mechanism is mainly passive diffusion. Forsythin is not the substrate of P-glycoprotein.