论文部分内容阅读
目的:建立芍药苷快速、灵敏的酶联免疫分析(ELISA)检测方法,并使用新方法检测中药材白芍中芍药苷的含量。方法:以制备出的芍药苷特异性单克隆抗体为基础,通过考察线性关系、精密度、回收率等方法学指标,建立芍药苷间接竞争酶联免疫分析(icELISA)方法,并应用此方法检测中药材白芍中的芍药苷含量。结果:该方法线性范围为3.9~250 ng·mL~(-1),板内和板间差异均不超过9.8%,平均回收率<110%,检测工作时间可控制在2 h内。采用该方法检测中药材白芍中芍药苷的含量,所得结果与HPLC一致。结论:建立的芍药苷icELISA方法,可为含芍药苷的微量生物样品检测、中药材及复方的质量控制分析提供更加快速灵敏的检测方法。
OBJECTIVE: To establish a rapid and sensitive enzyme-linked immunosorbent assay (ELISA) for the determination of paeoniflorin and to detect the content of paeoniflorin in Radix Paeoniae Alba by a new method. Methods: Paeoniflorin-specific monoclonal antibody was used as the basis to establish a method of indirect competitive enzyme-linked immunosorbent assay (icELISA) of paeoniflorin by examining the linear relationship, precision, recovery and other methodological indicators, and applied this method to detect Paeoniflorin in Chinese herb peony root. Results: The linear range of this method was 3.9-250 ng · mL -1. The intraplate and interplate differences were no more than 9.8% and the average recoveries were less than 110%. The detection time could be controlled within 2 h. The method was used to detect the content of paeoniflorin in Chinese white peony root. The obtained results were consistent with HPLC. CONCLUSION: The established method of icELISA of paeoniflorin can provide a more rapid and sensitive method for the quality control analysis of trace biological samples containing paeoniflorin, Chinese herbal medicines and compound prescriptions.