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目的 探讨用阴道毛滴虫可溶性抗原和单克隆抗体进行 EL ISA试验的条件。方法 选用不同抗原浓度(5 0μg/ m l～ 40 0μg/ mg)包被酶标板 ,选用不同孵育时间 (15 ～ 12 0 )、不同封闭剂 (脱脂奶粉、吐温 2 0 / PBS、牛血清白蛋白、小牛血清 )和不同酶标板 (国产和进口 )。结果 以 2 0 0 μg/ ml抗原量 ,用 1%牛血清白蛋白封闭 ,37℃孵育 1小时为本试验最佳条件。结论 检测 10例滴虫性阴道炎患者血清 ,阳性率为 90 % ,与多种寄生虫感染血清或单克隆抗体均无交叉反应。
Objective To investigate the conditions of ELISA for detecting Trichomonas vaginalis soluble antigen and monoclonal antibody. Methods Different concentrations of antigen (50μg / ml ~ 40μg / mg) were used to coat ELISA plates and incubated with different incubation time (15 ~ 120), different blocking agents (skimmed milk powder, Tween 20 / Protein, bovine serum) and different plates (domestic and imported). Results The optimal conditions for this experiment were 200 μg / ml antigen, blocked with 1% bovine serum albumin and incubated for 1 hour at 37 ° C. Conclusions The serum of 10 cases of trichomonas vaginitis was detected, the positive rate was 90%. There was no cross-reaction with serum or monoclonal antibodies of various parasites.