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目的:探索癌宁对人胃癌细胞的诱导凋亡作用。方法:采用体外细胞培养技术,通过形态学、分子生物学方法,对中药复方癌宁和顺铂诱导胃癌细胞凋亡进行了深入研究。结果:经癌宁及顺铂处理后的细胞出现凋亡形态学改变;癌宁在1 g/L浓度对细胞的凋亡诱导率是46.36％,与25 mg/L顺铂对细胞的凋亡诱导率49.12％间无显著差异(P>0.05),两者均与空白对照组有显著差异(P<0.01)。结论:癌宁及顺铂对人胃癌细胞具有诱导凋亡作用,中药复方癌宁可望成为一种有前途的新的癌细胞凋亡诱导剂。
Objective: To explore the apoptosis-inducing effect of Jianinging on human gastric cancer cells. METHODS: In vitro cell culture techniques were used to investigate the apoptosis of gastric cancer cells induced by Chinese herbal compounds Caining and Cisplatin through morphological and molecular biological methods. RESULTS: Morphological changes of apoptosis were observed in the cells treated with cisplatin and cisplatin. The induction rate of apoptosis was 46.36% at a concentration of 1 g/L in cancer and 25 mg/L cisplatin on apoptosis. There was no significant difference in the induction rate (49.12%) (P>0.05), both of which were significantly different from the blank control group (P<0.01). CONCLUSION: The cancer-canning and cisplatin have an apoptosis-inducing effect on human gastric cancer cells, and the traditional Chinese medicine compound carbendazim is expected to become a promising new apoptosis-inducing agent for cancer cells.