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目的 研究错配修复基因 (MMR)表达与食管癌和贲门癌癌变的关系和甲基苄基亚硝胺 (NMBzA)对其影响的研究。方法 采用多重逆转录聚合酶链反应 (RT -PCR)。结果 31例食管癌hMSH2基因表达明显降低占 2 2 .6 % ;hMLH1基因表达明显降低占 6 .45 % ;未发现hPMS2 ,GTBP和hPMS1基因表达降低。 1 1例食管癌旁组织分析 :hMSH2 ,hPMS2 ,GTBP ,hMLH1基因表达明显降低占 9.0 9% ;未发现hPMS1基因表达明显降低。 1 2例贲门癌组织分析 :hMSH2基因表达明显降低占 41 .7% ;GTBP ,hMLH1 ,hPMS1基因表达明显降低占 8.3 % ;未发现hPMS2基因表达明显降低。 8例贲门癌旁组织分析 :3例hMSH2基因表达明显降低 ;1例hPMS2基因表达明显降低 ;3例hMLH1基因表达明显降低 ;未发现GTBP ,hPMS1基因表达明显降低。对NMBzA致癌物诱发的人胎儿食管上皮癌细胞株分析发现 :hMLH1和hPMS1基因低表达 ;hMSH2 ,hPMS2 ,GTBP基因不表达。结论 食管癌和贲门癌癌变可能与错配修复基因失活有关 ;甲基苄基亚硝胺能引起错配修复基因表达异常。
Objective To study the relationship between mismatch repair gene (MMR) expression and the carcinogenesis of esophageal and cardiac cancers and the effect of methylbenzylnitrosamine (NMBzA) on it. Methods Multiplex reverse transcriptase polymerase chain reaction (RT-PCR) was used. Results The expression of hMSH2 gene in 31 cases of esophageal cancer was significantly reduced, accounting for 22.6%; the expression of hMLH1 gene was significantly decreased in 6.45%; the hPMS2, GTBP and hPMS1 gene expression was not reduced. An analysis of 11 cases of esophageal cancer adjacent tissues showed that the expression of hMSH2, hPMS2, GTBP, and hMLH1 genes was significantly reduced by 9.09%; hPMS1 gene expression was not significantly reduced. Analysis of 12 cases of cardiac cancer tissue: hMSH2 gene expression was significantly reduced by 41.7%; GTBP, hMLH1, hPMS1 gene expression was significantly reduced by 8.3%; hPMS2 gene expression was not significantly reduced. Analysis of 8 cases of cardiac cancer adjacent tissue: 3 cases of hMSH2 gene expression was significantly reduced; 1 case of hPMS2 gene expression was significantly reduced; 3 cases of hMLH1 gene expression was significantly reduced; no GTBP, hPMS1 gene expression was significantly reduced. Analysis of human fetal esophageal epithelial cancer cell lines induced by NMBzA carcinogens revealed that hMLH1 and hPMS1 genes were lowly expressed; hMSH2, hPMS2, and GTBP genes were not expressed. Conclusion The canceration of esophageal and cardiac cancers may be related to the inactivation of mismatch repair genes; methylbenzylnitrosamine can cause abnormal expression of mismatch repair genes.