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目的：探讨非生理条件对细胞凋亡的影响。方法：采用ＨＥ和ＤＮＡ染色的方法，在４℃组织培养条件下观察了小鼠胸腺实质内凋亡细胞的分布。结果：胸腺皮质、髓质内均有发生典型核形态改变的细胞，其细胞核的形态大小不一，具有这种核形态改变的细胞随体外培养时间的延长而增多。结论：体外非生理环境培养可诱导胸腺实质内各种细胞凋亡和坏死
Objective: To investigate the effect of non-physiological conditions on apoptosis. Methods: The distribution of apoptotic cells in thymus parenchyma of mice was observed under 4 ℃ culture conditions by HE staining and DNA staining. Results: There were typical nuclear morphological changes in the cortex and medulla of thymus. The morphological changes of nuclei varied with the time of in vitro culture. CONCLUSION: In vitro non-physiological culture can induce various apoptosis and necrosis in thymus parenchyma