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本研究利用本实验室开发的62个genic-SSR标记分析5个中国兰种的43个品种,其中52个标记呈多态性,其多态性指数(PIC)从0.054到0.824不等,平均为0.415,遗传多样性分析显示这43个材料之间的遗传距离平均为0.358,范围为0.016~0.600。对这些材料进行聚类分析和主成分分析,结果表明大多数来自一个物种的中国兰品种聚类在一起,而春兰分别聚类于几个系统树分支上,具有多起源性。与地理分布信息交叉分析发现:同一物种的品种往往聚类于一个大的系统树分支,但来自于不同地区的品种分别聚类于不同小分支;一些来自临近地域的品种往往聚类在一起,而这些品种有时不属于同一物种。这些结果说明遗传背景和地理环境存在一定的联系。
In this study, 43 genotypes of 5 Chinese blue species were analyzed using 62 genic-SSR markers developed in our laboratory. Among them, 52 markers were polymorphic and the polymorphic index (PIC) ranged from 0.054 to 0.824. The mean Was 0.415. Analysis of genetic diversity showed that the average genetic distance among these 43 materials was 0.358, ranging from 0.016 to 0.600. Cluster analysis and principal component analysis of these materials showed that most of the Chinese orchid cultivars from one species clustered together and Chunlan clustered on several phylogenetic branches and had multiple origins. Cross-analysis with geographic distribution information showed that varieties of the same species often clustered on a large branch of phylogenetic tree, but varieties from different regions clustered on different small branches respectively. Some varieties from adjacent regions often clustered together, And these species sometimes do not belong to the same species. These results indicate that there is a certain relationship between genetic background and geographical environment.