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使用蔗糖衬垫法提取了线粒体DNA(mtDNA),利用AFLP技术比较了滇一型水稻的4套不育系与保持系的mtDNA的差异。从92G03/92S09引物对选择扩增产物中得到了不育系与保持系差异条带。在约287bp处,保持系均有一条带,对应的不育系均缺少该条带。以上滇一型水稻不育系与保持系线粒体DNA差异的存在,表明可能与育性相关。
Mitochondrial DNA (mtDNA) was extracted using sucrose-lined method. The differences of mtDNA between four sterile lines and maintainer lines of Dian-1 type rice were compared using AFLP technique. Male sterile line and maintainer line were obtained from 92G03 / 92S09 primer pair. At about 287 bp, there was one band in the maintainer line, which was absent in the corresponding sterile lines. The presence of mitochondrial DNA differences in the Dian-1 type CMS lines and maintainers in Yunnan showed that fertility may be related to fertility.