采用香草醛-冰醋酸-高氯酸显色法,以齐墩果酸为对照,分光光度法测定急性子中总皂苷的含量。经显色后,齐墩果酸在541nm处有最大吸收,并在7.327—25.64μg/mL范围内吸光度与齐墩果酸量呈良好线性关系(r=0.9996),方法平均回收率为98.7%(n=6),RSD为0.51%。该方法简便、准确、灵敏,可以快速测定急性子中总皂苷的含量。 Vanillin - acetic acid - perchloric acid colorimetric method to oleanolic acid as a control, spectrophotometric determination of total saponin content of acute. The results showed that oleanolic acid had the maximal absorption at 541nm and the absorbance was linear with the amount of oleanolic acid (r = 0.9996) in the range of 7.327-25.64μg / mL. The average recovery was 98.7% (n = 6) with a RSD of 0.51%. The method is simple, accurate and sensitive, and can rapidly determine the content of total saponins in acute sub-species.