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L-精氨酸是一氧化氮主要的前体,已知细菌内毒素(脂多糖,LPS)刺激钠依赖性y~+载体的活力,后者通过白介素(IL)-1α和肿瘤坏死因子(TNF)-α等自分泌途径刺激猪肺动脉内皮细胞(PAEC)中L-精氨酸的运送。为了进一步证实LPS刺激NO的生成依赖于细胞因子自分泌的调节,自猪肺动脉制备内皮细胞,与LPS一起孵育,分析其上清液中IL-1α和TNF-α的生物活性,所用制剂有大肠杆菌127:B8内毒素、重组人体TNF-α(T-2410)、重组人体IL-1α(I-6764)、重组人体IL-1受体拮抗剂(IL-1ra)和纯化兔抗人体TNF IgG(TNF抗
L-Arginine is a major precursor of nitric oxide and bacterial endotoxin (lipopolysaccharide, LPS) is known to stimulate the activity of a sodium-dependent y- + vector that is activated by interleukin (IL) -1α and tumor necrosis factor TNF) -α and other autotransac- tive pathways stimulate the delivery of L-arginine in porcine pulmonary artery endothelial cells (PAECs). To further confirm that LPS-stimulated NO generation relies on the regulation of cytokine autocrine production, endothelial cells were prepared from porcine pulmonary artery and incubated with LPS to assay the biological activity of IL-1 alpha and TNF-alpha in their supernatants. The formulations used included large intestine Bacillus 127: B8 endotoxin, recombinant human TNF-α (T-2410), recombinant human IL-1α (I- 6764), recombinant human IL- 1 receptor antagonist (IL-1ra) and purified rabbit anti-human TNF IgG (TNF anti