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短串联重复序列（shor tandem repeat,STR）以核心序列重复单位数目的变化构成遗传多态性。在众多的STR基因座中,以三碱基重复或四碱基重复的STR基因座突变率低、稳定性好,且遵循孟德尔共显性遗传方式,是群体遗传学和法医遗传学高信息基因座的丰富来源[1]。本文参考有关文献[2-6],结合本实验室实验条件,筛选了6个STR座位进行扩增,并采用聚丙烯酰胺凝胶电泳及银染和（或）琼脂糖凝胶电泳EB染色进行带型分析,以多个同胞的STR片段长度等位片段,逆向推测其父母的相应四个位点,用于同胞间亲权鉴定。现报告如下。1 材料与方法
Short tandem repeats (STRs) constitute a genetic polymorphism with the number of repeat units in the core sequence. In many STR loci, STR loci with three or four base repeats have low mutation rates and good stability, and follow the Mendelian co-dominant heredity pattern, which is a high population of genetic and forensic genetics information Rich source of loci [1]. Reference to the relevant literature [2-6], combined with the laboratory experimental conditions, screening six STR loci for amplification and polyacrylamide gel electrophoresis and silver staining and (or) agarose gel electrophoresis EB staining Band-type analysis, with multiple siblings of STR fragment length allelic fragments, the reverse guess of the corresponding four parents of its parents, for siblings paternity testing. The report is as follows. 1 materials and methods