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比色分析是利用被测物在一定条件下借显色剂的作用形成有色化合物,然后测定溶液颜色深度的一种分析方法。它的定量分析理论基础是朗伯·比尔定律(A=log I_0/I=Kcb) (式中A为吸光度,K为比例常数,c为被测物浓度,b为液层厚度,若c以克分子浓度为单位,b以厘米为单位,K叫做克分子吸光系数,以ε表示)。比色分析按检测手段可分为目视比色、光电比色和分光光度法。随着仪器的发展和普及,目前多
Colorimetric analysis is the use of analytes under certain conditions by the role of the developer to form a colored compound, and then determine the color depth of an analytical method. Its theoretical basis for quantitative analysis is the Lambert-Beer law (A = log I_0 / I = Kcb) (where A is the absorbance, K is the constant of proportionality, c is the concentration of the analyte, b is the thickness of the liquid layer, Molecular concentration units, b in centimeters, K is called molecular extinction coefficient, ε). Colorimetric analysis by detection means can be divided into visual colorimetric, photoelectric colorimetric and spectrophotometric methods. With the development and popularization of instruments, many are currently available