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[目的]研究超重处理对茎瘤芥(Brassica juncea var.tumida Tsen et Lee)种子萌发及幼苗生长的影响,探讨超重处理对茎瘤芥种子生长发育的影响机理。[方法]以0、2 000、3 000和4 000 r/min的超重力分别处理茎瘤芥种子1.5、3.0和4.5 h后,进行培养,观察其出苗时间、出苗率、性状表现,并测定幼苗叶绿素含量、可溶性蛋白含量和过氧化物酶活性。[结果]与对照相比,超重处理后,茎瘤芥发芽率、发芽势和发芽指数随处理条件变化有不同程度的降低。超重处理后,苗期茎瘤芥植株变矮,根系变短且发达,叶长变短,鲜重和干重都有不同程度的降低,而茎粗则不同程度增加。超重处理后,茎瘤芥幼苗叶绿素含量和过氧化物酶活性都有不同程度的增加,而可溶性蛋白含量有不同程度的降低。超重处理可以影响茎瘤芥幼苗的生长,处理时间以及重力加速度是影响其生长的重要因素,适当的超重处理可以促进植物生长。同时,超重处理可增强茎瘤芥幼苗的抗倒伏能力、抗有害物的能力和合成有机物的能力。[结论]该研究可为超重技术在茎瘤芥生产中的应用提供参考依据。
[Objective] The research aimed to study the effects of overweight treatment on seed germination and seedling growth of Brassica juncea var. Tumida Tsen et Lee and to explore the mechanism of overweight treatment on the growth and development of tumorous stem mustard seed. [Method] After treated with supergravity of 0, 2 000, 3 000 and 4 000 r / min for 1.5, 3.0 and 4.5 h, respectively, the seedlings were inoculated with the supernatant at 0, 2, 000, 3 000 and 4 000 r / min for observation of their emergence, emergence and traits. Seedling chlorophyll content, soluble protein content and peroxidase activity. [Result] Compared with the control, the germination rate, germination energy and germination index of tumorous stem mustard decreased with different treatment conditions after overweight treatment. After overweight treatment, the seedlings of Tumstatin decreased, the root system became shorter and developed, the leaf length became shorter, the fresh weight and dry weight decreased to some extent, while the stem diameter increased to some extent. After overweight treatment, the chlorophyll content and peroxidase activity of the stem mustard seedlings increased to some extent, while the content of soluble protein decreased to some extent. Overweight treatment can affect the growth of tumorous mustard seedlings, treatment time and gravitational acceleration are important factors that affect their growth. Appropriate overweight treatment can promote plant growth. At the same time, overweight treatment can enhance the lodging resistance of tumorous mustard seedlings, anti-pest ability and the ability to synthesize organic matter. [Conclusion] This study may provide a reference for the application of overweight technology in the production of tumorous mustard.