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目的了解医院感染多耐药鲍氏不动杆菌(MDR-ABA)的耐药基因及亲缘性。方法采用PCR对20株MDR-ABA进行β-内酰胺酶基因、氨基糖苷类耐药基因、转座子遗传标记、整合子遗传标记共36种耐药基因的检测,并以耐药基因作为分子标志作聚类分析;同时用脉冲场凝胶电泳(PFGE)进行分子同源性检测以作参照。结果 20株MDR-ABA中,TEM、ADC、OXA-23群阳性率分别为100%、75%、50%,而其他β-内酰胺酶相关基因均为阴性;aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6′)-Ⅰb、ant(3″)-Ⅰ阳性率分别为10%、15%、30%、25%;转座子遗传标记tnpU检出率为55%、整合子遗传标记qacE△1-sul1阳性15株(75%),并对检测结果作样本聚类分析,聚类分析提示20株MDR-ABA可分为A、B两群,均存在克隆传播现象;而PFGE法通过图形条带分析所得结果与聚类分析结果具有一定差异。结论 MDR-ABA耐药基因检出率较高,并可导致医院内克隆传播感染,聚类分析比PFGE更能反映菌株遗传信息,是分析同源性更科学方法。
Objective To understand the multidrug-resistant Acinetobacter baumannii (MDR-ABA) resistance genes and their relationship in hospital. Methods A total of 36 MDR-ABA genes were detected by polymerase chain reaction (PCR), including β-lactamase gene, aminoglycoside resistance gene, transposon genetic marker and integron genetic marker. Markers for clustering analysis; at the same time using pulsed-field gel electrophoresis (PFGE) for molecular homology detection as a reference. Results The positive rates of TEM, ADC and OXA-23 in 100 MDR-ABA strains were 100%, 75% and 50%, respectively, while all the other β-lactamase related genes were negative. The aac (3) (3) -Ⅱ, aac (6 ’) - Ⅰb and ant (3 ") - Ⅰ were 10%, 15%, 30% and 25% respectively. The detection rate of transposon tnpU was 55% The results showed that qACE △ 1-sul1 was 15 (75%) positive in the integron, and the results were clustered by cluster analysis. Cluster analysis indicated that 20 MDR-ABA strains could be divided into A and B groups, , While the results of PFGE were different from the results of cluster analysis by the graphic strip analysis.Conclusion The detection rate of MDR-ABA resistance gene is high and can lead to the spread of infection in the hospital, and cluster analysis can reflect more than PFGE Genetic information of strains is a more scientific method of analyzing homology.