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本文应用体外连续双室灌流系统观察了在灌流液中含雌二醇(E_2)和不含E_2时,克罗米芬对雌性大鼠中基底下丘脑(MBH)释放促黄体激素释放激素(LRH)和垂体释放促黄体激素(LH)的影响。实验选用220～250gWistar系间情期雌性大鼠,在早8点用颈椎脱位处死,取出MBH和垂体后分别放入连续双室灌流系统的MBH室和垂体室,然后用199液进行灌流,灌流液从MBH室到垂体室,自垂体室收集流出液,每隔10分钟0.5ml。在此基础上用含E_2和不含E_2的199液连续灌流加克罗米芬
In this paper, we observed the effect of clomiphene on the release of luteinizing hormone releasing hormone (LRH) and the release of luteinizing hormone releasing hormone (LHH) from basal hypothalamus (MBH) in female rats by using the continuous dual chamber perfusion system Effect of pituitary releasing luteinizing hormone (LH). Experimental use of 220 ~ 250g Wistar interspace female rats, as early as 8 points with cervical dislocation of death, remove the MBH and pituitary, respectively, into the continuous double chamber perfusion system MBH room and pituitary room, and then with 199 liquid perfusion From the MBH chamber to the pituitary gland, collect the effluent from the pituitary gland at 0.5 ml every 10 minutes. On this basis, continuous infusion of clomiphene citrate with 199 liquid containing E 2 and no E 2