论文部分内容阅读
酶活力-速率法测定酶促反应中底物或产物随时间的变化量。该方法每隔一定时间(10～60s)测定底物或产物的变化量,在线性期内确定每分钟平均吸光度值(△A/min),并计算出酶活力单位。该方法要求分析仪器具有恒温装置和自动监测功能。半自动生化分析仪和微量流动比色计均能满足这些
Enzyme activity - rate method for the determination of enzymatic reaction substrate or product changes over time. The method determines the change of the substrate or product at regular intervals (10-60s), determines the average absorbance value per minute (ΔA / min) during the linear period, and calculates the unit of enzyme activity. The method requires an analytical instrument with a thermostat and automatic monitoring. Semi-automatic biochemical analyzer and micro-flow colorimeter can meet these