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目的:研究重楼皂苷Ⅱ(PPⅡ)对人乳腺癌细胞的作用及影响机制。方法:MTT法检测PPⅡ对乳腺癌Bcap37、MCF-7、MDA-MB-231、MDA-MB-453细胞增殖抑制作用,流式细胞仪检测PPⅡ对Bcap37和MDA-MB-231细胞周期分布和细胞凋亡的影响,Hoechst 33342染色观察细胞核变化,Western blotting检测蛋白表达。结果:PPⅡ对乳腺癌Bcap37、MCF-7、MDA-MB-231、MDA-MB-453细胞的增殖均具有抑制作用,并呈剂量依赖性;流式分析细胞周期分析PPⅡ阻滞Bcap37细胞在G2/M期,MDA-MB-231细胞在S期和G2/M期,细胞凋亡率呈浓度和时间依赖性;Hoechst33342显示细胞凋亡特征;Western blotting检测凋亡相关蛋白Cleaved-Caspase9和Cleaved-PAPR增多。结论:PPⅡ对乳腺癌细胞系具有增殖抑制作用,其机制可能与引起G2/M期阻滞和促进细胞凋亡有关。
Objective: To study the effect and mechanism of PP Ⅱ on human breast cancer cells. Methods: The inhibitory effect of PPⅡ on the proliferation of Bcap37, MCF-7, MDA-MB-231 and MDA-MB-453 cells was detected by MTT assay. The cell cycle distribution of Bcap37 and MDA- Apoptotic changes were observed by Hoechst 33342 staining. The protein expression was detected by Western blotting. Results: PPⅡinhibited the proliferation of Bcap37, MCF-7, MDA-MB-231 and MDA-MB-453 cells in a dose-dependent manner. Flow cytometry analysis showed that PPⅡ blocked Bcap37 cells in G2 / M phase. The apoptosis rate of MDA-MB-231 cells in S phase and G2 / M phase was in a concentration- and time-dependent manner. The apoptosis characteristics of Hoechst33342 cells were detected by Western blotting. The apoptosis-related proteins Cleaved-Caspase9 and Cleaved- PAPR increased. Conclusion: PPⅡ can inhibit the proliferation of breast cancer cell lines, and its mechanism may be related to the arrest of G2 / M phase and the promotion of cell apoptosis.