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【目的】分析以Thatcher为遗传背景的小麦抗叶锈病近等基因系的遗传多样性,探讨SRAP技术在小麦抗叶锈病基因标记及基因克隆研究中应用的可行性。【方法】采用SRAP(sequence-related amplified polymorphism)技术对23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系和感病对照Thatcher进行分析。【结果】从128对引物中筛选得到41对具有多态性引物,每个引物组合产生6～41个多态性条带,共产生537个多态性条带,多态性条带率达49.5%,平均每个引物组合产生13.1个多态性条带;每个引物组合产生1～13个特异性条带,共产生115个特异性条带,特异性条带率为10.6%,平均每个引物组合产生2.8个特异性条带。聚类分析将23个小麦抗叶锈病近等基因系和Thatcher在相似系数0.72处分为A、B两大类,其中96%的个体归入B类。B类又分为Ⅰ、Ⅱ两个亚类,95%的个体聚在第Ⅱ亚类。第Ⅱ亚类在相似系数为0.785附近分为4小类。【结论】23个小麦抗叶锈病近等基因系间存在一定的差异。SRAP技术是一种简单有效的分子标记系统,可在小麦叶锈病抗病基因的研究中应用。
【Objective】 The genetic diversity of near-isogenic lines of leaf rust resistance in wheat with Thatcher as a genetic background was analyzed and the feasibility of SRAP in the application of wheat leaf rust-resistant gene markers and gene cloning was discussed. 【Method】 Twenty-three near-isogenic lines of wheat leaf rust resistance and that of susceptible controls, Thatcher, were analyzed by using sequence-related amplified polymorphism (SRAP). 【Result】 From the 128 pairs of primers, 41 pairs of polymorphic primers were screened, and each primer combination produced 6 ~ 41 polymorphic bands, producing a total of 537 polymorphic bands, the rate of polymorphic bands reached 49.5%, and the average primer combinations produced 13.1 polymorphic bands; each primer combination produced 1 ~ 13 specific bands, a total of 115 specific bands were generated, the specific banding rate was 10.6%, the average Each primer combination produced 2.8 specific bands. Cluster analysis of 23 wheat leaf rust near isogenic lines and Thatcher at the similarity coefficient 0.72 into A, B two categories, of which 96% of individuals classified as B class. Category B is divided into two subclasses I and II, and 95% of the individuals clustered in the second subclass. The second sub-category is divided into four sub-categories near the similarity coefficient of 0.785. 【Conclusion】 There are some differences in near-isogenic lines among 23 wheat leaf rust resistance cultivars. SRAP is a simple and effective molecular marker system that can be used in the research of disease resistance genes of wheat leaf rust.