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目的：克隆表达具有重要生物功能的人单核细胞趋化蛋白１（ｈｕＭＣＰ－１）。方法：从人外周血单核细胞（ＰＢＭＣ）中提取 ｐｏｌｙ（Ａ）~＋ ＲＮＡ，用 ＲＴ－ＰＣＲ法扩增出 ｈｕＭＣＰ－１ｃＤＮＡ。将 ｈｕＭＣＰ－１ｃＤＮＡ插入融合表达载体 ｐＧＥＸＩＮ， １ｍｍｏｌ／Ｌ异丙基－β－Ｄ－硫代半乳糖苷（ＩＰＴＧ）诱导后获得高效表达。结果： Ｗｅｓｔｅｒｎ ｂｌｏｔ证明与 ｈｕＭＣＰ－１单克隆抗体有明显交叉反应。结论：ｈｕＭＣＰ－１基因的克隆并表达成功为今后的实验室和临床研究提供了有用的材料。
AIM: To clone and express human monocyte chemotactic protein 1 (huMCP-1) with important biological functions. Methods: Poly (A) ~ + RNA was extracted from human peripheral blood mononuclear cells (PBMCs) and the huMCP-1 cDNA was amplified by RT-PCR. The huMCP-1 cDNA was inserted into the fusion expression vector pGEXIN and induced by 1mmol / L isopropyl-β-D-thiogalactoside (IPTG). Results: Western blot showed significant cross-reaction with huMCP-1 monoclonal antibody. Conclusion: The successful cloning and expression of huMCP-1 gene provides useful materials for future laboratory and clinical studies.