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EB病毒在流行病学上与鼻咽癌癌变密切有关。 应用体外转染法,将EBV—W(YH)片段导入RatI细胞,在20天时出现细胞交叉重叠生长的转化灶。经软琼脂生长筛选后,用分子杂交方法可在转化细胞DNA内检测到W片段的存在,转化细胞接种裸鼠后可生长肿瘤,并在肿瘤组织DNA中再次检测到EBV—W(YH)片段的存在,转化细胞染色体的原位杂交分析提示,在有些中期相染色体上有银粒出现。 以上结果说明,EBV—W(YH)基因片段已转染至细胞内,并能在大鼠细胞内复制,与肿瘤形成呈正相关,它可能对细胞的依赖生长起着重要作用。
Epstein-Barr virus is epidemiologically closely related to the carcinogenesis of nasopharyngeal carcinoma. Using in vitro transfection method, EBV-W (YH) fragments were introduced into Rat I cells and cross-overlapped growth foci appeared on day 20. After soft agar growth and screening, the presence of W fragment in the DNA of the transformed cells can be detected by molecular hybridization. After the transformed cells were inoculated into nude mice, tumors could be grown and EBV-W (YH) fragments were detected again in the tumor tissue DNA. The presence of chromosomes and in situ hybridization analysis of transformed cells suggest that silver particles appear on some metaphase chromosomes. The above results show that the EBV-W (YH) gene fragment has been transfected into cells and can replicate in rat cells and is positively correlated with tumor formation. It may play an important role in cell-dependent growth.