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脑啡肽是内源性鸦片样肽类物质,是神经递质和调制物质,有调制痛觉等多种功能。近年来,发现该物质也存在于血液循环中,与心血管调节、呼吸等有着密切关系。上海市高血压研究所在国内首次建立了测定人血浆亮氨酸—脑啡肽(LEK)样物质的放射免疫法。人血浆脑啡肽量微且可被酶迅速裂解,测定困难。本法用甲状腺球蛋白作为EK 载体,碳二亚胺作为连接剂,采用小剂量(每只200μg)背部皮内多点法免疫家兔,每月强化注射(每只100μg),笫3
Enkephalins are endogenous opioid peptides, neurotransmitters and modulators, modulate pain and many other functions. In recent years, it has been found that the substance is also present in the blood circulation, which is closely related to cardiovascular regulation and respiration. Shanghai Institute of Hypertension in China for the first time established a human plasma leucine - enkephalin (LEK) -like radioimmunoassay. Human plasma enkephalin micro-and can be rapidly lysis of enzymes, determination of difficulties. This law with thyroglobulin as EK carrier, carbodiimide as a linker, a small dose (each 200μg) back intradermal multipoint immunization of rabbits, monthly intensive injection (each 100μg), Zi 3