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目的研究二硫化碳(CS2)经皮染毒对小鼠骨髓细胞及精子细胞的毒性作用。方法选择无特异性病原体级健康成年雄性NIH小鼠50只,采用完全随机的方式分为5个组,分别为:CS2高剂量组[900mg/kg,即1/2半数致死浓度(LD50)]、中剂量组(360mg/kg,即1/5LD50)、低剂量组(180mg/kg,即1/10LD50),阴性对照组(生理氯化钠)和阳性对照组(环磷酰胺150mg/kg),采用封闭涂皮法染毒,每天1次,每周5d,直至实验结束。于第6天处死部分小鼠进行骨髓细胞微核检测,于第35天处死其余小鼠进行精子畸形实验,显微镜下观察并计数微核及畸形精子数。结果CS2中、高剂量组骨髓细胞微核数高于阴性对照组,差异具有统计学意义(P<0.05),同时低、中、高剂量组精子畸形率高于阴性对照组,差异具有统计学意义(P<0.05)。结论一定剂量的CS2经皮染毒对雄性小鼠的骨髓细胞及精子细胞具有一定的损伤作用,提示CS可能具有致突变作用。
Objective To study the toxic effect of CS2 transdermal drug on mouse bone marrow cells and sperm cells. Methods Fifty healthy adult male NIH mice without specific pathogen were randomly divided into five groups: CS2 high dose group [900 mg / kg (LD50)] , Low dose group (180mg / kg or 1 / 10LD50), negative control group (physiological sodium chloride) and positive control group (cyclophosphamide 150mg / kg) , Using a closed coating method of exposure, once a day, 5days a week, until the end of the experiment. On the 6th day, some mice were sacrificed to detect the micronuclei of bone marrow cells. The remaining mice were sacrificed on the 35th day for sperm deformity test. The numbers of micronuclei and deformity sperm were observed under a microscope. Results The number of micronuclei in bone marrow cells of CS2 high dose group was higher than that of negative control group (P <0.05), and the sperm deformity rate of low, medium and high dose group was higher than that of negative control group, the difference was statistically significant Significance (P <0.05). Conclusion A certain dose of CS2 transdermally penetrates on the bone marrow cells and sperm cells of male mice with a certain degree of damage, suggesting that CS may have a mutagenic effect.