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目的研究子宫内膜异位症组织中孕激素受体亚型的表达,探讨其在内膜异位症发生、发展和治疗中的意义。方法选取2005-01-2005-05山东大学齐鲁医院妇产科22例卵巢子宫内膜异位囊肿的囊壁组织,6例同时行子宫切除术患者的在位内膜组织和13例正常内膜组织,采用RT-PCR技术对孕激素受体(PR-A+B)和孕激素受体B亚型(PR-B)的基因表达进行半定量研究。结果巧克力囊肿组织中PR-A+B和PR-BmRNA表达显著低于正常内膜组织和子宫内膜异位症(EM)患者在位内膜组织(P<0.01);巧克力囊肿组织中PR-B/PR-A+BmRNA的比值低于正常子宫内膜组织PR-B/PR-A+BmRNA的比值,差异有显著性(P<0.01)。结论PR-A+B和PR-B/PR-A+BmRNA的比值降低可能与EM发生、发展及治疗中“孕激素耐受”相关。
Objective To study the expression of progesterone receptor subtypes in endometriosis and to explore its significance in the occurrence, development and treatment of endometriosis. Methods From January 2005 to May 2005, 22 cases of ovarian endometriosis cyst in obstetrics and gynecology department of Shandong Qilu Hospital, 6 cases of eutopic endometrium and 13 cases of normal endometrium Tissue, semi-quantitative RT-PCR was performed on the gene expression of progesterone receptor (PR-A + B) and progesterone receptor subtype B (PR-B). Results The expression of PR-A + B and PR-B mRNA in chocolate cyst was significantly lower than that in normal endometrium and endometriosis (EM) patients (P <0.01). The expression of PR- The ratio of B / PR-A + B mRNA was lower than the ratio of PR-B / PR-A + B mRNA in normal endometrium (P <0.01). Conclusion The decrease of the ratio of PR-A + B to PR-B / PR-A + BmRNA may be related to the occurrence, development and treatment of progesterone in EM.