Aim:To develop an efficient RNA interference system using phagemid particles displaying the epidermal growth factor (EGF) ligand. Methods:pSilencer 1.0-siEGFP and pSilencer4.1-siAkt plasmids were constructed by gene clone technology. The modified helper phage genome (plasmid) M 13KO7EGFCT was used to pack-age phagemids, such as pSilencer 1.0-siEGFP and pSilencer4.1-siAkt. ELISA was used to quantify the titer of the progeny virus particles. Single-strand DNA was extracted and analyzed by agarose gel electrophoresis to evaluate the percentage of the phagemid particles. The expression level of the reporter gene enhanced green fluorescence protein (EGFP) was determined by transducing phagemid par-ticles packaging pSileneer 1.0-siEGFP into cells. The level of Akt gene expression in cells transduced phagemid particles packaging pSilencer4. 1-siAkt was exam-ined by Weste blotting. Hydroxycamptothecin (HCPT) was used to enhance the gene transduction efficiency. Results:RNAi vectors pSilencer1.0-siEGFP and pSilencer4.1-siAkt were successfully constructed. Phagemid-encoding siRNA can be packaged efficiently. After the cells were infected by EGF displaying phagemid particles in the presence of HCPT, the expression of the target gene EGFPor Akt was substantially downregulated. Conclusion:Cell-targeted phagemid particles are efficient siRNA delivery vectors in the presence of HCPT.