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采用普通小麦农大3338和京冬6号的组合构建的包含216个株系的DH系为材料,以包含379个标记的高密度遗传连锁图谱为基础,利用复合区间作图法,通过一年两点田间试验,对株高及其组成成分不同节间长度的QTL进行分析。结果表明,一年两点最终株高共定位到8个QTL,分布在染色体2D,4B,4D,5A,6D,7A上,共解释株高变异为91.86%(北京)、92.63%(临汾)。各节间表型数据总共定位到28个QTL,分布在染色体2B,2D,3B,4A,4B,4D,5A,6A,6D,7A上。这些QTL基本包括了影响最终株高的8个位点,各节间长度还有部分特有的QTL。上述结果为在育种中实现对株高、穗下节长和其他节间长度的精细遗传操作及深入解析株高性状形成的遗传学基础提供了理论依据。
Based on the high-density genetic linkage map containing 379 markers, DH lines with 216 lines constructed by the combination of common wheat Nongda 3338 and Jingdong 6 were used as materials for the study. Field trials were conducted to analyze the QTLs for plant height and its internode length with different composition. The results showed that there were 8 QTLs located at chromosomes 2D, 4B, 4D, 5A, 6D, and 7A on the chromosomes at the end of one year, which explained 91.86% (Beijing) and 92.63% (Linfen) . A total of 28 QTLs were mapped across the internode phenotypes on chromosomes 2B, 2D, 3B, 4A, 4B, 4D, 5A, 6A, 6D, 7A. These QTLs basically included eight sites that affected the final plant height, with some unique QTLs in each internode length. The above results provide a theoretical basis for the genetic manipulation of plant height, spike length and other internode length in breeding and further analysis of the genetic basis of plant height traits.