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公认的阿片受体拮抗剂纳洛酮对阿片类药物具有肯定的逆转效应。为实验其对非阿片类麻醉药的药理作用如何影响及相互作用机制,我们采用膜片钳技术的吸附式构型和内面向外式构型在单通道水平研究了纳洛酮与依托咪酯和Kca通道的作用。材料与方法无菌条件下取新生1～3天SD大鼠皮质进行神经元体外原代培养。膜片钳实验选用对称性高钾溶液,选择培养4～8天具典型形态的活神经元进行实验。加药前先记录下—60mV～+60mV间以10mV间隔的各钳制电位下的单通道电流作为对照,然后加实验药物,2分钟后再在各对应电压下采样。资料用Pclamp(6.0.2)程序软件(Axon Instruments Burliagme CA)分析,显著性检验采用配对t检验和方差分析。
The accepted opioid receptor antagonist naloxone has a positive reversal effect on opioids. In order to test how they affect the pharmacological effects of non-opiate anesthetics and their interaction mechanisms, we investigated the effects of naloxone and etomidate on the single- And Kca channels. MATERIALS AND METHODS: Cultured SD rat cortical neurons cultured in vitro for 1 to 3 days under aseptic conditions. Patch clamp experiments using symmetrical high-potassium solution, choose to cultivate 4 to 8 days with typical morphology of living neurons experiments. Before dosing, record the single-channel current under the clamping potentials of -60mV ~ + 60mV and 10mV intervals as the control, then add the experimental drugs, and then sample them under the corresponding voltage after 2 minutes. Data were analyzed using Pclamp (6.0.2) program software (Axon Instruments Burliagme CA), paired t-test and analysis of variance were used for significance test.