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为研究高三尖杉酯碱（Ｈｏｍ）对白血病细胞分化和肿瘤转移的影响．采用四氮唑蓝法检测ＨＬ－６０细胞的分化及测定游离磷含量观测肿瘤细胞Ｎａ＋－Ｋ＋－ＡＴＰ酶和Ｍｇ＋＋－ＡＴＰ酶活性．肿瘤转移模型采用小鼠皮下接种Ｌｅｗｉｓ肺癌观测肺转移灶数和小鼠足跖接种Ｐ３８８白血病细胞观察动物生存期，且用γ射线测定法评价１２５Ｉ－纤维蛋白原与肿瘤细胞的结合能力．结果显示Ｈｏｍ１．５～４．５μｇ／Ｌ能诱导ＨＬ－６０细胞分化，Ｈｏｍ０．１μｍｏｌ／Ｌ２４ｈ对Ｆｒｉｅｎｄ白血病细胞Ｎａ＋－Ｋ＋－ＡＴＰ酶活性抑制，对Ｍｇ＋＋－ＡＴＰ酶活性无影响，Ｈｏｍ０．５～１．５ｍｇ·ｋｇ－１／ｄ剂量下能抑制Ｌｅｗｉｓ癌肺转移和Ｐ３８８白血病细胞淋巴道转移，以及Ｈｏｍ２～１０ｍｇ／Ｌ抑制纤维蛋白原与癌细胞的结合．提示Ｈｏｍ能通过以上途径诱导肿瘤细胞分化和抗肿瘤转移
To study the effects of homoharringtonine (Hom) on leukemia cell differentiation and tumor metastasis. The tyrosine blue method was used to detect the differentiation of HL-60 cells and the content of free phosphorus was measured to observe the activity of Na+-K+-ATPase and Mg++-ATPase in tumor cells. Tumor metastasis model The mice were inoculated subcutaneously with Lewis lung cancer to observe the number of lung metastases and the mice were inoculated with P388 leukemia cells to observe the survival period of the animals. The binding ability of 125I-fibrinogen and tumor cells was evaluated by γ-ray assay. The results showed that Hom 1.5 ~ 4.5μg/L can induce HL-60 cell differentiation, Hom0.1μmol/L 24h on Friend leukemia cells Na + - K + - ATPase activity inhibition, no effect on Mg++ - ATPase activity, Hom0.5 ~ The dose of 1.5mg·kg-1/d could inhibit Lewis lung metastasis and lymph node metastasis of P388 leukemia cells, and Hom2～10mg/L inhibited the binding of fibrinogen and cancer cells. Prompt that Hom can induce tumor cell differentiation and antitumor metastasis through the above pathways