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用简便的方法获得大量的花粉植株,仍然是当前水稻单倍体育种的主要研究问题之一。Sunderland 曾用液体培养菸草花药成功地获得了大量的胚状体和花粉植株。我们曾试验过用三角烧瓶垫纱布或滤纸加入培养液接种水稻花药,经培养也获得花粉愈伤组织。用液体培养水稻花药的试验已观察到有较好的效果,现简报如下:
Using simple methods to obtain a large number of pollen plants is still one of the main research problems in current haploid breeding in rice. Sunderland has successfully obtained large numbers of embryoid bodies and pollen plants with liquid cultured tobacco anthers. We have tried to use triangular flask pad gauze or filter paper to join the culture medium inoculated rice anthers, pollen callus obtained by culture. Experiments with liquid anther culture of rice have observed good results and are summarized below: