论文部分内容阅读
目的分析2011年-2012年安徽省B型流感病毒流行病学特点及血凝素基因变异情况。方法收集安徽省2011年-2012年B型流感阳性标本,病毒分离培养后选取16株对其HA基因扩增并测序。结果 14株Victoria系与2009年-2012年北半球疫苗株B/Brisbane/60/2008相比,核苷酸同源性在98.5%~99.5%之间,氨基酸同源性在95.4%~99.3%之间;2株Yamagata系与疫苗株B/Wisconsin/01/2010相比,核苷酸同源性为99.3%,氨基酸同源性为99.3%,仅有1个~4个抗原位点发生变化,未出现新的变异株。结论 2011年-2012年安徽省以B型Victoria系为流行株,未发现明显变异,WHO推荐的疫苗株B/Brisbane/60/2008起到积极的预防作用。
Objective To analyze the epidemiological characteristics of influenza B virus in Anhui province from 2011 to 2012 and the genetic variation of hemagglutinin gene. Methods The positive samples of influenza B from 2011 to 2012 in Anhui province were collected. After isolating and culturing, 16 strains of HA were amplified and sequenced. Results Compared with the northern hemisphere vaccine strain B / Brisbane / 60/2008 from 2009 to 2012, the 14 Victoria strains had nucleotide homology of 98.5% -99.5% and amino acid homology of 95.4% -99.3% The homology between two strains of Yamagata was 99.3% compared with that of vaccine strain B / Wisconsin / 01/2010. The homology of amino acids was 99.3%, only 1 ~ 4 antigen sites changed, No new variant appeared. Conclusion There was no obvious variation in the type B Victoria strain from 2011 to 2012 in Anhui Province. The vaccine strain B / Brisbane / 60/2008 recommended by the WHO plays an active preventive role.