1980年wood建立了自胎盘分离单核巨噬细胞的方法。本文对该法作了改进,以便为上述细胞的体外研究提供较为稳定丰富的细胞来源,并对细胞进行签别。将胎盘绒毛膜组织切成2—3毫米的小块,置无Ca~(2+)、mg~(2+)的Dulbecco氏PBS-液(pH7.4)中充分洗涤,除去血清和红细胞,再用PBS~-配制的0.25%胰蛋白酶、0.1g/ml胶原酶和0.1mg/ml的脱氧核糖核酸酶的混合液消化(如用脱氧核糖核 In 1980 Wood established a method of separating mononuclear macrophages from the placenta. In this article, the method is improved to provide a more stable and abundant source of cells to cells in vitro and to sign the cells. The placental chorionic tissue was cut into 2-3 mm pieces and washed thoroughly with Dulbecco’s PBS-solution (pH 7.4) without Ca 2+, Mg 2+, to remove serum and erythrocytes, Then digested with a mixture of 0.25% trypsin, 0.1 g / ml collagenase and 0.1 mg / ml deoxyribonuclease prepared by PBS ~ - (as with deoxyribonucleic acid