在致畸试验中,动物是否受孕是很重要的环节,而交配前的诱发动情期及不同雌雄比例,对动物受孕又有一定的影响。Whitten主张交配前应进行动情周期的诱发和同步。美国的致畸试验标准操作程序(Sops)规定雌雄比例为1:1。Wilson认为雌雄比例可以2:1或3:2,本实验研究将为提高致畸试验中的动物受孕率提供参考依据。 In teratogenicity test, whether or not the animals are pregnant is an important link, and the pre-mating induced estrus and different male and female ratio have certain influence on the animal’s pregnancy. Whitten advocates that estrus cycles should be initiated and synchronized before mating. The United States teratogenic test standard operating procedures (Sops) provides for male and female ratio of 1: 1. Wilson believes that male-female ratio can be 2: 1 or 3: 2, this experimental study will provide a reference for improving the rate of pregnancy in teratogenic experiments.