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作者利用放射性同位素标记的乳糖被大肠菌群发酵产生放射性CO_2的原理,提供了一种大肠菌群快速鉴定的方法。在一无菌试管中(9.5×1.5厘米)加入下列物质:~(14)C-乳糖0.1毫升(1微居里/毫升,20微居里/微克分子),水样(或滤膜)0.1毫升,营养肉汤含有2×10~(-5)M乳糖;0.001%溴酚兰(pH 6.0～6.2)0.2毫升。试管立即用带有聚乙烯中心导管的橡皮塞子密封,置于37℃水浴中震荡。在要求的时间,于塑料中心导管内注入0.2毫升氢氧化铵以吸收二氧化碳,再继续培养15分
Using the principle that radioactive isotope labeled lactose is fermented by coliform bacteria to produce radioactive CO 2, the authors provide a rapid method for the identification of coliforms. The following were added to a sterile test tube (9.5 x 1.5 cm): ~ (14) C-lactose 0.1 ml (1 microCi / ml, 20 microColi / micromolar), water sample Ml, nutrient broth containing 2 × 10 ~ (-5) M lactose; 0.001% bromophenol blue (pH 6.0 ~ 6.2) 0.2 ml. The test tube was immediately sealed with a rubber stopper with a polyethylene central catheter and placed in a 37 ° C water bath for shaking. At the required time, 0.2 ml of ammonium hydroxide was injected into the plastic center catheter to absorb the carbon dioxide, and the culture was continued for another 15 minutes