论文部分内容阅读
2,2,6,6-Tetramethyl-4-(bis-ethyteneimino) triazinamino-piperidine-1-oxyl was used as the spin label. In ESR spectra of the product obtained (SL-DNA), the strongly immobilized peak greatly prevailed over the weakly immobilized component and a significant difference existed between their microwave saturation characteristics. The spin labeling reaction was investigated in some details. With the aid of beat denaturation (100℃, 15 min) of SL-DNA in conjunction with acid degradation (pH 1.5, 37℃, 23h), the quantity of labels bound to DNA was determined. Namely, the number of labels bound to 1000 nucleotides was found to be 4.0—12.6. The structural transition temperature of SL-DNA attained from variable temperature measurements was about 63℃ and a rise in this temperature was observed with increasing salt concentration.
The ESBL spectra of the product obtained (SL-DNA), the strongly immobilized peak greatly prevailed over the weakly immobilized component and a significant difference existed between their microwave saturation characteristics. The spin labeling reaction was investigated in some details. With the aid of beat denaturation (100 ° C, 15 min) of SL-DNA in conjunction with acid degradation 1.5, 37 ° C, 23h), the quantity of labels bound to DNA was determined. Namely, the number of labels bound to 1000 nucleotides was found to be 4.0-12.6. The structural transition temperature of SL-DNA attained from variable temperature measurements was about 63 ° C and a rise in this temperature was observed with increasing salt concentration.