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目的 :研究不同浓度的三氧化二砷 (As2 O3 )对恶性黑色素瘤人A3 75细胞和小鼠B16细胞的凋亡诱导作用 ,为As2 O3 治疗恶性黑色素瘤提供新的理论和实验依据。方法 :用流式细胞术 (FCM )检测A3 75细胞和B16细胞的凋亡诱导及杀伤作用 :用不同剂量的As2 O3 (0 .1mmol·L-1、0 .2 5mmol·L-1和 0 .5mmol/L-1) ,对小鼠进行腹腔注射 (10ml·kg-1,连续注射 10d后 ,检测小鼠血清与心、肝、肾有关的生化指标。结果 :As2 O3 浓度分别为 5 μmol·L-1、10 μmol·L-1和 2 0 μmol·L-1时 ,A3 75和B16细胞的凋亡率分别为 11.85 %、5 5 .5 1%、5 4 .90 %和 9.81%、2 6 .4 5 %、9.93% :As2 O3 诱导A3 75和B16细胞总的凋亡和坏死率分别为11.90 %、5 5 .71%、5 7.4 0 %和 12 .96 %、2 6 .99%、87.13% ;不同剂量的As2 O3 (0 .1mmol·L-1～ 0 .5mmol·L-1)对小鼠肝、肾和心肌等功能无明显影响 ,与对照组各项指标无显著性差异 (P >0 .0 5 )。结论 :不同浓度的As2 O3 能明显诱导恶性黑色素瘤A3 75及B16细胞凋亡和坏死 ,对A3 75细胞的凋亡诱导作用强于B16细胞。As2 O3 (0 .1mmol·L-1～ 0 .5mmol·L-1)对小鼠肝、肾和心肌等功能无明显损伤
OBJECTIVE: To study the apoptosis-inducing effects of different concentrations of As2O3 on malignant melanoma A375 cells and mouse B16 cells, and to provide new theoretical and experimental evidences for the treatment of malignant melanoma with As2 O3. Methods: The apoptotic induction and killing effect of A375 cells and B16 cells were detected by flow cytometry (FCM): As2O3 (0.1 mmol·L-1, 0.55 mmol·L-1 and 0 (5ml / L, 5mmol / L) .The mice were injected intraperitoneally (10ml · kg-1) for 10d and the serum, heart, liver and kidney were detected.Results: The apoptosis rates of A375 and B16 cells were 11.85%, 55.1%, 54.9% and 9.81% respectively at L-1, 10 μmol·L-1 and 20 μmol·L-1, , 24.4% and 9.93% respectively. The total apoptosis and necrosis rates of A3 75 and B16 cells induced by As 2 O 3 were 11.90%, 57.5%, 57.4% and 12.96%, respectively. 99% and 87.13% respectively. There was no significant effect of different doses of As 2 O 3 (0.1 mmol·L -1 ~0.5 mmol·L -1) on liver, kidney and myocardium in mice (P0.05) .Conclusion: As2O3 can obviously induce the apoptosis and necrosis of A375 and B16 cells, and induce the apoptosis of A375 cells more strongly than that of B16 cells.As2O3 (0 .1mmol·L-1 ~ 0.5mmol·L-1) had no effect on the liver, kidney and myocardium in mice Damage