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今运用83年建立的新生小白鼠(刚出生1～2天)十二指肠,在无菌条件下切下0.2～0.3mm进行体外培养。设培前空白对照组和培养组。选用多杯法器官培养,培养液为 RPMI 1640加 10％小牛血清,青霉素100μg/ml,再加链霉素100μg/ml、庆大霉素50μg/ml,再加Hepes缓冲液,用碳酸氢钠调培液pH至7.2;培养组分别加健脾中药(黄芪、茯苓、白术、甘草、广木香、山药制成针剂,浓度为1:1),分别稀释成10种浓度(mg/ml):2.5;3.125;6.25;12.5;18.75;25
The newborn duodenal (born 1 to 2 days after birth) duodenum established in 83 years is used for in vitro culture under sterile conditions with 0.2-0.3 mm cut. Set culturing before the blank control group and culture group. Use a multi-cup method for organ culture. The culture broth was RPMI 1640 plus 10% calf serum, penicillin 100 μg/ml, streptomycin 100 μg/ml, gentamycin 50 μg/ml, and Hepes buffer with bicarbonate. Sodium inoculum solution pH to 7.2; culture group were added spleen Chinese medicine (Astragalus, Poria, Atractylodes, licorice, broad wood, yam made injections, the concentration of 1:1), were diluted to 10 concentrations (mg/ml ): 2.5; 3.125; 6.25; 12.5; 18.75; 25