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目的：研究用ＨＳＶ－１载体经猴嗅神经通路将外源基因导入中枢神经系统的方法。方法：将ＨＳＶ－１载体接种于猴嗅神经末梢，在不同时间取各部位脑组织检测ｌａｃＺ基因表达产物β－半乳糖苷酶活性，并观察猴脑组织局部及全身的病理学和免疫学变化。结果：接种ＨＳＶ－１载体后４ｄ在嗅中枢皮质神经元内检测到β－半乳糖苷酶活性，并持续１个月以上；脑组织及全身脏器未见病理学及免疫学改变。结论：ＨＳＶ－１载体可通过嗅神经通路将外源基因导入中枢神经系统并在其中表达，
OBJECTIVE: To study the method of introducing exogenous gene into the central nervous system by monkey olfactory nerve pathway with HSV-1 vector. Methods: The HSV-1 vector was inoculated on the olfactory nerve endings of monkey and the expression of β-galactosidase in lacZ gene was detected at different time points. The pathological and immunological changes of local and systemic monkey brain were observed . Results: β-galactosidase activity was detected 4 days after inoculation of HSV-1 vector in the olfactory central cortex neurons for more than 1 month; pathological and immunological changes were not observed in brain tissue and whole body organs. Conclusion: The HSV-1 vector can be introduced into the central nervous system through the olfactory nerve pathway and expressed in the central nervous system,