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目的在金黄地鼠口腔癌模型中确定血卟啉单甲醚荧光光谱对口腔黏膜癌的特异性诊断的可行性。方法选用成年金黄地鼠建立口腔黏膜癌模型,用9,10-二甲基1,2苯并蒽涂布于颊囊表面10周(n=20),小鼠处死前,组织中注射血卟啉单甲醚溶液,通过波长405nm的光纤光谱仪记录黏膜组织的药物荧光光谱,选用光谱参数S作为诊断标准,测量荧光光谱后,处死动物,对需测量的黏膜组织进行染色并记录组织病理学评价结果。结果 HMME染色的光谱显示正常组黏膜无明显染色,癌症组黏膜被染成不同程度的变化,两者呈现显著差异;癌性组织的S值(140≤S≤200)均高于正常组织(S≤10),且具有统计学意义。结论通过血卟啉单甲醚荧光光谱特异性荧光光谱可以作为诊断口腔黏膜恶性病变的一个标准。
Objective To determine the feasibility of fluorescence spectroscopy of hematoporphyrin monomethyl ether in the diagnosis of oral mucosal carcinoma in the golden hamster oral cancer model. Methods The model of oral mucosal carcinoma was established in adult golden hamster. The surface of cheek pouch was treated with 9,10-dimethyl-1,2-benzanthracene for 10 weeks (n = 20). Before the mice were sacrificed, blood was injected into the tissues The solution of mucosal tissue was stained with 405nm wavelength spectrophotometer and the mucosal fluorescence spectrum of the mucosa was recorded by using a spectrofluorometer with a wavelength of 405nm. The spectral parameters of S were selected as the diagnostic criteria. After the fluorescence spectra were measured, the animals were sacrificed and the mucosal tissues to be measured were stained and histopathological evaluation result. Results The HMME staining showed no obvious staining in mucosa of normal group. The mucosa of cancer group was stained to varying degrees, which showed significant difference. S value (140≤S≤200) in cancerous tissues was higher than that in normal tissues ≤10), and has statistical significance. Conclusion The fluorescence spectra of hematoporphyrin monomethyl ether can be used as a criterion for the diagnosis of malignant oral mucosa.