论文部分内容阅读
对本室筛选的高活性Ｂ．ｔ．野生株进行了ｃｒｙ 基因检测，Ｓｏｕｔｈｅｒｎ杂交证明ｃｒｙＩＡｃ和ｃｒｙ１Ｃ位于质粒上．利用接合转移和电穿孔转化等方法对这些菌株进行了遗传改良研究．结果表明：高效野生株７４０４、ＨＤ－１－Ｘ和９５１０ 不易获得并表达外源ｃｒｙ 基因，而高效野生株Ｂｔｉ． ｔ－１８９７ 的电转化频率较高，并获得以Ｂｔｉｔ－１８９７ 为受体，对甜菜夜蛾、棉铃虫、黏虫和淡色库蚊均有毒力，具有ｃｒｙＩＡｃ、ｃｒｙＩＣ、ｃｒｙＩＶ及ｃｙｔ多价基因的广谱工程菌株．
High-activity screening of our room. t. The cry gene was detected in wild strain, and Southern blotting confirmed that cryIAc and cry1C were located on the plasmid. These strains were genetically modified by conjugation transfer and electroporation transformation. The results showed that highly efficient wild-type strain 7404, HD-1-X and 9510 were not easy to obtain and express foreign cry genes, while wild-type Bti. t-1897 had a higher frequency of electroporation and obtained the Btit-1897 receptor, which was toxic to beet armyworm, cotton bollworm, armyworm and Culex pipiens pallens, with cryIAc, cryIC, cryIV and cyt polyvalent genes Broad spectrum engineering strains.