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目的调查二连浩特口岸鼠体寄生蜱种群组成及自然感染病原体情况。方法从捕获的鼠体上收集寄生蜱,提取单只蜱DNA基因组及总RNA,采用PCR或RT-PCR方法检测莱姆病螺旋体、Q热立克次体、巴贝西原虫、斑点热立克次体、无形体、森林脑炎病毒、布尼亚病毒、克里米亚-刚果出血热病毒。结果共采集鼠体寄生蜱152只,分别为草原血蜱、银盾革蜱和亚东璃眼蜱,其中草原血蜱为优势种,占捕获总数的98.03%。从草原血蜱、银盾革蜱和亚东璃眼蜱中检测到斑点热立克次体19份,检出率为12.50%,其他病原体检测均为阴性。结论二连浩特口岸地区存在斑点热立克次体自然疫源地。
Objective To investigate the population composition of parasitic ticks and the pathogens of naturally infected mice in Erenhot Port. Methods The parasitized ticks were collected from the captured mice and DNA genomes and total RNA of single ticks were collected. The Borrelia burgdorferi, Q fever bacterium, Second body, Anaplasma, Forest encephalitis virus, Bunia virus, Crimean-Congo hemorrhagic fever virus. Results A total of 152 parasitic ticks were collected, which were grassland blood-swallow, silver-bermudagris and oriental apple, respectively. Among them, the dominant species was blood-storphax, accounting for 98.03% of the total. Nineteen spotted hot rickettsiae were detected in the bloodworts, silver moth and ticks, and the detection rate was 12.50%. All the other pathogens were negative. Conclusion There are some natural foci of Spotted fever ricketts in the port area of Erenhot.