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目的:探讨结肠癌患者组织中microRNA-99a表达水平以及对结肠癌细胞增殖和迁移的影响。方法:取南京医科大学附属常州二院胃肠病中心49例结肠癌患者肿瘤组织、癌旁组织(癌旁5cm)标本以及结肠癌细胞HCT-116、HT-29、SW-480、Caco-2和正常结肠上皮细胞HCoe Pic,采用实时荧光定量PCR检测结肠癌患者肿瘤组织和结肠癌细胞中microRNA-99a表达水平;结肠癌细胞株HT-29转染microRNA-99a抑制剂后,CCK-8法检测microRNA-99a对结肠癌细胞增殖的变化;transwell法观察microRNA-99a对结肠癌细胞迁移的影响;Western blotting检测了HT-29中FGFR-3的表达水平。结果:microRNA-99a表达在结肠癌组织中明显高于癌旁组织(6.27±0.48 vs 1.34±0.54,P<0.05)、在肿瘤细胞中明显高于正常结肠上皮细胞(5.48±0.34,7.67±0.24,5.78±0.22,6.28±0.44 vs 1.45±0.37,P<0.05)。转染microRNA-99a抑制剂后,HT-29细胞的增殖和迁移能力均明显下降(P<0.05);同时,HT-29中FGFR-3显著降低(P<0.05)。结论:microRNA-99a在结肠癌组织中高表达,低表达microRNA-99a可减弱结肠癌细胞的增殖和迁移能力,且可能通过FGFR-3信号通路发挥作用。
Objective: To investigate the expression of microRNA-99a in colon cancer tissue and its effect on the proliferation and migration of colon cancer cells. METHODS: Tumor specimens from 49 patients with colon cancer and adjacent tissues (5cm paracancer) of colon cancer and colon cancer cells HCT-116, HT-29, SW-480 and Caco-2 were collected from the gastrointestinal center of Changzhou Second Hospital affiliated to Nanjing Medical University. The expression of microRNA-99a in colon cancer cells and colon cancer cells was detected by real-time fluorescence quantitative PCR. After colon cancer cell line HT-29 was transfected with microRNA-99a inhibitor, CCK-8 method The effect of microRNA-99a on colon cancer cell migration was observed by transwell method. The expression of FGFR-3 in HT-29 was detected by Western blotting. Results: The expression of microRNA-99a in colon cancer tissues was significantly higher than that in adjacent non-cancerous tissues (6.27 ± 0.48 vs 1.34 ± 0.54, P <0.05), significantly higher than that in normal colorectal epithelial cells (5.48 ± 0.34, 7.67 ± 0.24 , 5.78 ± 0.22, 6.28 ± 0.44 vs 1.45 ± 0.37, P <0.05). After transfection with microRNA-99a inhibitor, the proliferation and migration ability of HT-29 cells were significantly decreased (P <0.05). Meanwhile, FGFR-3 in HT-29 was significantly decreased (P <0.05). Conclusion: MicroRNA-99a is overexpressed in colon cancer tissues. The low expression of microRNA-99a attenuates the proliferation and migration of colon cancer cells, and may play a role through the FGFR-3 signaling pathway.