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目的：研究重组人肿瘤坏死因子α（ＴＮＦ－α）对放烧复合伤小鼠骨髓有核细胞（ＢＭＮＣ）ＩＬ－３Ｒ的调节作用。方法：以放烧复合伤小鼠为模型，观察一次性静脉注入不同剂量的ＴＮＦ－α对ＢＭＮＣ增殖的影响及１０万ＵＴＮＦ－α对ＩＬ－３Ｒα表达及功能、ＢＭＮＣ数量的调节作用。结果：①静注不同剂量的ＴＮＦ－α对放烧复合伤小鼠ＢＭＮＣ增殖的影响不一；②１０万Ｕ的ＴＮＦ－α１、３ｄ上调放烧复合伤小鼠ＩＬ－３Ｒα的平均含量（对阳性率及功能无明显影响），而致ＢＭＮＣ数量明显降低；７ｄ时恢复至对照组水平；③１０万ＵＴＮＦ－α１、３ｄ致放烧复合伤小鼠ＢＭＮＣ计数明显降低。结论：１０万ＵＴＮＦ－α在体内虽能上调放烧复合伤小鼠ＩＬ－３Ｒα的平均含量而剌激造血，但可能因其同时激活造血调控网络整体效应却抑制骨髓造血。
Objective: To investigate the regulatory effect of recombinant human tumor necrosis factor-α (TNF-α) on bone marrow nucleated cells (BMNC) IL-3R induced by radiation and burn in mice. Methods: The effects of single intravenous infusion of different doses of TNF-α on the proliferation of BMNC and the regulation of the expression of IL-3Rα by 10 000 UTNF-α and the number of BMNC were observed in burn-burn combined injury model mice. Results: ①The effects of different doses of TNF-α on the proliferation of BMNC in burn-burn injury mice were different; ② The average content of IL-3Rα in burn-burn injury mice was increased by 100000 U TNF-α1,3d Rate and function had no significant effect), while the number of BMNC was significantly decreased; on the 7th, the level of BMNC was restored to that of the control group; CONCLUSIONS: Although 1 000 000 UTNF-α can up-regulate the average level of IL-3Rα in mice after burn-combined injury, it may stimulate hematopoiesis, but may inhibit bone marrow hematopoiesis by activating the overall effect of hematopoietic regulation network.