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LncRNA MIR4435-2HG is characterized as an oncogene in lung cancer.However,its role in ovarian carcinoma (OC) is unclear.In this study,we aimed to investigate the role of MIR4435-2HG in OC.We found that both MIR4435-2HG and transforming growth factor beta 1 (TGF-β1) were upregulated in OC.MIR4435-2HG is associated with tumor metastasis but not with tumor size.Upregulation of MIR4435-2HG distinguished early stage (Stage Ⅰ and Ⅱ) OC patients from healthy controls.Correlation analysis showed that plasma levels of MIR4435-2HG and TGF-β1 were positively correlated only in OC patients,qPCR and weste blot analysis results showed that MIR4435-2HG overexpression led to upregulation of TGF-β1 in OC cells,while TGF-β1 treatment did not significantly affect MIR4435-2HG expression.Transwell invasion and migration assays showed that MIR4435-2HG and TGF-β1 promoted the invasion and migration of OC cellswhile TGF-β inhibitor suppressed the invasion and migration of these cells.Further analysis of the Transwell invasion and migration assay results showed that TGF-β inhibitor reduced the effects of MIR4435-2HG overexpression.Therefore,our results suggested that IncRNA MIR4435-2HG may promote OC by upregulating TGF-β1.Further characterization of the functions of MIR4435-2HG in OC may provide novel targets for cancer therapies.