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采用高效液相色谱内标法定量测定茄呢醇的含量.采用4.6mm×25cm硅胶柱;V(正己烷)/V(异丙醇)=100:1的混合液为流动相,流速为1.0mL/min;维生素D为内标,紫外检测器检测,检测波长为210nm;ABS为0.04.样品检测线性范围为9～30μg;相关系数r=0.9992;回收率91.9%—102.4%;精密度RSD=2.93%.改进后的色谱条件检测范围宽,灵敏度高,重现性好.
The content of solanesol was determined by high performance liquid chromatography with internal standard method.The mobile phase consisted of 4.6 mm × 25 cm silica gel column and a mixture of V (n-hexane) / V (isopropanol) = 100: 1 1.0 mL / min; vitamin D was the internal standard, UV detector detection, detection wavelength was 210nm; ABS was 0.04. The linear range of the sample was 9 ~ 30μg; correlation coefficient r = 0.9992; recovery 91.9% -102.4%; precision RSD = 2.93%. The improved chromatographic conditions have a wide range of detection, high sensitivity and good reproducibility.