论文部分内容阅读
本文用放射配体结合测定法观察了硫酸锌对５－ＨＴ３受体激动剂５－ＨＴ与［３Ｈ］ｇｒａｎｉｓｅｔｒｏｎ竞争ＮＧ１０８－１５细胞中５－ＨＴ３受体能力的影响。结果证实，硫酸锌（１ｍＭ）能使５－ＨＴ竞争受体的浓度──反应曲线左移，半数抑制结合浓度降低（Ｐ＜０．０５），对斜率无明显影响。提示锌离子可能也影响５－ＨＴ３受体功能，有增强５－ＨＴ与５－ＨＴ３受体结合的作用。
Radioligand binding assays were used to investigate the effect of zinc sulfate on the 5-HT3 receptor agonist 5-HT and [3H] granisetron competing for 5-HT3 receptors in NG108-15 cells. The results confirmed that zinc sulfate (1mM) can make 5-HT competitive receptor concentration ─ ─ reaction curve shifted to the left, half inhibit the binding concentration decreased (P <0.05), no significant effect on the slope. Tip zinc ions may also affect the 5-HT3 receptor function, and enhance 5-HT and 5-HT3 receptor binding.