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目的探讨一种同时测定唇齿清胃丸中黄芩苷和盐酸小檗碱含量的高效液相色谱法。方法以Kromasil100-5C18柱(4.6 mm×150.0 cm,5μm)为色谱柱;以乙腈为流动相A,以0.05 mol/L磷酸二氢钾溶液为流动相B,采用梯度洗脱;检测波长278 nm,柱温30℃,流速为1.0 ml/min。结果盐酸小檗碱进样量在0.0124~0.3100μg,进样量0.0217~0.5425μg范围内有良好的线性关系,平均回收率分别为99.1%、99.04%,RSD分别为0.94%、0.85%(n=6)。结论该方法准确可靠专属性强、结果稳定,重复性好。
Objective To explore a method for the simultaneous determination of baicalin and berberine hydrochloride in Lipou Qingwei Pills by high performance liquid chromatography. Methods Kromasil 100-5C18 column (4.6 mm × 150.0 cm, 5 μm) was used as the chromatographic column. Acetonitrile was used as mobile phase A, and 0.05 mol / L potassium dihydrogen phosphate solution was used as mobile phase B. The gradient was 278 nm The column temperature was 30 ℃ and the flow rate was 1.0 ml / min. Results The linear range of berberine hydrochloride injection was 0.0124 ~ 0.3100μg with the average recoveries of 99.1%, 99.04% and RSD 0.94%, 0.85% (n = 6). Conclusion The method is accurate, reliable and specific, with stable results and good repeatability.