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目的评价急性脑梗死患者血清中toll样受体4(TLR4)和核转录因子(NF-κB)信号通路水平与脑血流量的相关性。方法选取在本院接受治疗的100例大脑中动脉(MCA)供血区域脑梗死患者作为试验组,再选取同期接受体检健康的100例正常人作为对照组,采用免疫组织化学方法检测2组患者的TLR4和NF-κB水平,分析2组研究对象的大脑前动脉(ACA)、MCA收缩期血流峰值与TLR4、NF-κB的相关性。结果试验组ACA血流峰值(55.54±4.22)cm/s高于对照组(48.65±3.54)cm/s(t=9.145,P<0.001),而试验组MCA血流峰值(132.45±5.65)cm/s低于对照组(141.54±3.54)cm/s(t=8.436,P<0.001)。试验组TLR4水平(4.29±0.46)ng/m L及NF-κB水平(144.43±23.67)ng/m L均高于对照组TLR4(3.65±0.57)ng/m L及NF-κB(115.63±24.45)ng/m L(t=5.861、5.677,P=0.012、0.0018);TLR4及NF-κB水平与ACA血流速度呈正相关,与MCA血流速度呈负相关(P<0.05)。结论急性脑梗死患者MCA、ACA血流峰值与TLR4及NF-κB水平存在相关性。
Objective To evaluate the correlation between serum levels of toll-like receptor 4 (TLR4) and nuclear factor-kappa B (NF-κB) signaling and cerebral blood flow in patients with acute cerebral infarction. Methods A total of 100 patients with cerebral infarction in the middle cerebral artery (MCA) supplying area undergoing treatment in our hospital were selected as the experimental group, and 100 normal subjects receiving the same physical examination at the same period were selected as the control group. Immunohistochemistry TLR4 and NF-κB levels were measured. The relationship between ACA and MCA peak TLR4 and NF-κB in two groups were analyzed. Results The ACA blood flow peak (55.54 ± 4.22) cm / s in the experimental group was significantly higher than that in the control group (48.65 ± 3.54 cm / s, t = 9.145, / s lower than the control group (141.54 ± 3.54) cm / s (t = 8.436, P <0.001). The level of TLR4 (4.29 ± 0.46) ng / m L and the level of NF-κB (144.43 ± 23.67) ng / m L in the experimental group were significantly higher than those in the control group (3.65 ± 0.57 ng / m L and NF-κB 115.63 ± 24.45 ) ng / m L (t = 5.861,5.677, P = 0.012,0.0018). The levels of TLR4 and NF-κB were positively correlated with ACA blood flow velocity and negatively correlated with MCA blood flow velocity (P <0.05). Conclusion The peak of MCA and ACA in patients with acute cerebral infarction have correlation with the levels of TLR4 and NF-κB.