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本文在前期苗叶瘟抗谱测定的基础上,通过对空间诱变粤香占和青华占高代品系(6代以上)病圃田间穗颈瘟的鉴定,表明苗期表现抗稻瘟病的品种多数在成熟期同样表现抗或高抗稻瘟病。SSR多态性分析表明粤香占和青华占突变品系与原种间存在1.6%～17.4%和7.2%～10.4%的多态性频率,并存在3种多态性表现形式:(1)扩增片段数增加,(2)扩增片段数减少,(3)扩增片段大小有差异。说明由空间环境诱导的DNA变异可能并非是单纯的点突变。进一步分析诱变品系的多态性频率与抗病性的关系,发现部分诱变品系的抗性随着多态性频率的增加而提高。
Based on the preliminary determination of leaf blast resistance in early stage, the identification of panicle blast in the field of high-yielding lines (more than 6 generations) The majority of cultivars also showed resistance or resistance to blast at maturity. SSR polymorphism analysis showed that there were 1.6% -17.4% and 7.2% -10.4% of the polymorphic frequencies in Guangdong and Zhanhua, and there were three polymorphic forms: (1) The number of amplified fragments increases, (2) the number of amplified fragments decreases, and (3) the amplified fragment sizes differ. This suggests that DNA variation induced by space environment may not be a mere point mutation. Further analysis of mutagenic lineages of the relationship between the frequency of polymorphism and disease resistance and found that some of the mutagenic strains with the increase in the frequency of polymorphism increased.