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目的：改良９９Ｔｃｍ－ＷＢＣ制备条件并评价其特性．方法：取１０ｍｌ家兔及人全血分离ＷＢＣ，用９９Ｔｃｍ－ＨＭＰＡＯ介导标记，评价ＷＢＣ分离率、９９Ｔｃｍ－ＷＢＣ的标记率、稳定性、活性及其在家兔脓肿模型和炎性肠病患者体内的炎症定位特性．结果：ＷＢＣ分离率达７０％，９９Ｔｃｍ－ＷＢＣ标记率和活性率分别高达６０％和９０％．放射性不断自ＷＢＣ中逸脱．注射９９Ｔｃｍ－ＷＢＣ后１０ｍｉｎ家兔大腿脓肿即显影，４ｈ时最清晰．９９Ｔｃｍ－ＷＢＣ可清晰地显示活动性炎性肠病受累肠段部位和范围．结论：１０ｍｌ血可分离和制备出满足临床显像要求的９９Ｔｃｍ－ＷＢＣ．
Objective: To improve the preparation conditions of 99Tcm-WBC and evaluate its characteristics. Methods: WBCs were isolated from 10ml rabbit and human whole blood and labeled with 99Tcm-HMPAO to evaluate the isolation rate of WBC, the labeling rate, stability and activity of 99Tcm-WBC and its clinical significance in rabbit models of abscess and inflammatory bowel disease In vivo inflammatory localization. Results: The isolation rate of WBC was 70%, and the labeling rate and activity of 99Tcm-WBC were as high as 60% and 90% respectively. Radioactivity continuously escaped from WBC. 10min injection of 99Tcm-WBC rabbit thigh abscess that is developing, 4h clearest. 99Tcm-WBC clearly shows active bowel involvement and extent of inflammatory bowel disease. Conclusion: 10ml blood can be separated and prepared to meet the clinical imaging requirements of 99Tcm-WBC.