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目的构建间隙连接蛋白43(Cx43)和绿色荧光蛋白(GFP)双基因共表达重组腺病毒载体。方法利用 PCR方法从真核表达载体 pcDNA3.0-Cx43扩增 Cx43片断,利用 DNA 重组技术,将目的基因 Cx43克隆至含有报告基因GFP 的穿梭质粒 pAdTrack-CMV,然后在 BJ5183细胞中将重组穿梭质粒 pAdTrack-Cx43与 pAdeasy-1质粒进行同
Objective To construct recombinant adenovirus vector co-expressing gap junction protein 43 (Cx43) and green fluorescent protein (GFP) genes. Methods The Cx43 fragment was amplified by PCR from the eukaryotic expression vector pcDNA3.0-Cx43. The recombinant plasmid was cloned into the shuttle plasmid pAdTrack-CMV containing the reporter gene GFP by DNA recombination technique. The recombinant shuttle plasmid pAdTrack-Cx43 is the same as pAdeasy-1 plasmid